PhD student position: deciphering the molecular basis of allostery in CRISPR associated proteins using single-molecule experiments
PhD student position: deciphering the molecular basis of allostery in CRISPR associated proteins using single-molecule experiments
The Biomolecular Nano-Dynamics group at the Dept. of Chemistry, University of Basel is looking for a highly talented and motivated PhD student with a background in (bio-)physics, or biophysical chemistry to join our team for the study of allosteric mechanisms governing recently discovered CRISPR associated proteins using novel techniques, such as single-molecule fluorescence/FRET and nanopore trapping.
Your position
This PhD project will grow with you. You will create time-resolved direct observations of single CARF proteins - aka. CRISPR suicide proteins - undergoing allosteric activation, substrate processing, deactivation, etc. How this works, on what timescales, by which conformational rearrangements has not been observed yet in real-time, leaving the underlying molecular processes obscure. In this PhD project, you'll be able to solve (some of) these nanoscale puzzles. Steps involved in the project:
- Single-molecule fluorescence/FRET/DyeCycling experiments
- Solid-state nanopore/NEOtrap experiments
- Advanced data analysis, coding, kinetic modeling
- Protein production and functionalization
Your profile
Prior experience in several of these areas is desired:
Essential requirements:
- Single-molecule experiments and interpretation
- Advanced data analysis, coding, modeling, machine learning
- Optics and fluorescence microscopy and/or nanopore techniques
- Protein biochemistry
Essential requirements:
- MSc degree in physics or (bio-)chemistry with excellent grades
- High motivation and curiosity about fundamental questions on the physical-chemistry of life
- Independent analytical thinking and systematic work style
- Excellent experimental problem-solving skills and perseverance
- Friendly team player proficient in English communication (speaking & writing)
We offer you
- An exciting and fully funded PhD project in Basel, Switzerland
- Embedding in a productive, interdisciplinary group with an open and interactive lab culture
- State-of-the-art research infrastructure and facilities
- A highly international and interdisciplinary setting stimulating your professional and personal development
- The GRACE graduate center, wide-ranging sports offers, etc.
- A beautiful and very livable city at the intersection of Germany, France, and Switzerland
Application / Contact
Applications are invited before/by April 30th, 2024. Please submit your application via Write an email with subject 'Application PhD4 Firstname Lastname', and include:
your letter of motivation, your CV, and email addresses of 2-3 references - all merged into one single pdf file named 'Application PhD4 Firstname Lastname.pdf'.
For questions, please contact Prof. Sonja Schmid: Write an email.
Applications are invited before/by April 30th, 2024. Please submit your application via Write an email with subject 'Application PhD4 Firstname Lastname', and include:
your letter of motivation, your CV, and email addresses of 2-3 references - all merged into one single pdf file named 'Application PhD4 Firstname Lastname.pdf'.
For questions, please contact Prof. Sonja Schmid: Write an email.
Universität Basel
4000 Basel
4000 Basel
Adresse
Universität Basel
Über die Firma
